Hafidzi, Aimi Syaeirah
(2012)
Archiving expressed mRNA transcripts by using CapFinder method / Aimi Syaeirah Hafidzi.
Degree thesis, Universiti Teknologi MARA (UiTM).
Abstract
Therefore, to have a better cDNA and to improve the construction of the cDNA libraries it is recommended to use cap structure at the end of mRNAs strand for isolation of the full-length mRNAs as to develop more full-strength sequences of genes. CapFinder, CapJumping and CapSelect are some of the techniques used to get a better extraction of the mRNA. In this experiment, reverse transcriptase enzyme that will be used is Moloney Murine Leukemia Viruses reverse transcriptase (MMLV RTase).
Item Details
| Item Type: | Thesis (Degree) |
|---|---|
| Creators: | Creators Email / ID Num. Hafidzi, Aimi Syaeirah UNSPECIFIED |
| Contributors: | Contribution Name Email / ID Num. Thesis advisor Mohd Yusoff, Rosmadi UNSPECIFIED |
| Subjects: | R Medicine > RS Pharmacy and materia medica > Materia medica > Pharmaceutical chemistry |
| Divisions: | Universiti Teknologi MARA, Shah Alam > Faculty of Pharmacy |
| Programme: | Bachelor of Pharmacy (Hons.) |
| Keywords: | Reverse transcription; polymerase chain reaction; CapFinder; mRNA; molecular biology |
| Date: | 2012 |
| URI: | https://ir.uitm.edu.my/id/eprint/65541 |
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