Determination of xylanase activity from fermentation of oil palm frond / Nurhamieza Md Huzir

Oil palm frond was one of the disposal residues in oil palm industry. Oil palm frond can be potentially used as a substrate for lignocellulolytic enzyme production. In this experiment, grinded oil palm frond was used as a substrate in solid state fermentation using Aspergillus fumigatus to produce CMCase and Xylanase enzyme. The independent variables in this experiment were the fermentation time and the water treatment during fermentation. Experiment was run for 3,5,7,9 and day 11. Further investigation was continued to analyze the effect of water fed to the production of reducing sugar, Xylanase and CMCase
activity. Maximum production ofreducing sugar was 33.725μmole at day 5 in the presence of water. Both Xylanase and CMCase produced highest activity on the
11th day of experiment without water fed which were 1. 13 and 0.497 U/ml respectively. Fermentation with water fed increased the moisture content and yielded more reducing sugar. Xylanase and CMCase were optimum in absence of
water fed due to acidic condition of fermentation. Statistical analysis showed that water treatment do not have significant correlation towards reducing sugar
production. Meanwhile, t test proved that water fed strategy do not affect the Xylanase but have an interaction towards CMCase.