Chemical constituents from the leaves of Jasminum sambac and its antioxidant potential / Nurul Fatin Fakhira Mohd Padli

Antioxidant is a substance which can impede the oxidation of other molecules. It
plays an important role in impeding free radicals from damaging the skin and
harmful ultraviolet rays. Traditional dietary supplement from Jasminum sambac
extract is not very demanding in the market due to lack of further analysis of
antioxidant in J. sambac. Apart from that, antioxidant potential of J. sambac may
differ due to physical factors such as temperature, humidity, nutrients content in
soil and air movement. J. sambac is also known as Arabian Jasmine originates from
Oleaceae family. The objective of the study is to determine chemical constituents
and antioxidant properties of leaves of J. sambac. Preliminary screening showed
the presence of tannins, cardiac glycoside, steroid and saponins. Essential oil was
obtained by hydrodistillation of J. sambac leaves mainly containing nonanal and 2 buten-
I-one. DPPH assay was applied to determine the antioxidant activities of
crude extract. Dichloromethane (DCM) extract showed a moderate scavenging with
IC50 of 6.24 ppm compared to ascorbic acid as standard which showed highest
scavenging effect with IC50 of 2.90 ppm. Methanol and petroleum ether extract
showed weak scavenging activity with IC50 greater than 100 ppm. Hence, DCM
extract is the most antioxidative extract compared to methanol and petroleum ether
extracts. Phytochemical test for crude extracts mainly showed the presence of
terpenoid, aromatic and antioxidative compound. Hence, benzophenone was found
as antioxidative compound in dichloromethane extract by GC-MS and its
characteristic were confirmed by FTIR and TLC analysis of crude extract.