Evaluation of antioxidant and antimicrobial potency of stem of entada spiralis / Nur Khairunnisa' Maha Yudden

Entada spiralis known as 'Beluru' or 'Sintok' which is from Leguminosae family
was used in this research to determine the antioxidant and antimicrobial properties.
This plant was known to contain chemical substances which are phytochemical or
also known as secondary metabolites and have been used to treat disease and as
health care. In determining antioxidant and antimicrobial properties, several methods
were conducted. The stem of E.spiralis was extracted using four different polarities
of solvents such as petroleum ether, dicholoromethane, ethyl acetate and methanol.
Phytochemical screening on TLC using certain spraying reagent, FTIR analysis, dot
blot assay, radical scavenging activity and disc diffusion method was conducted to
evaluate antioxidant and antimicrobial potency of E.spiralis. From the phytochemical
screening test, it has been screened that stem of E.spiralis contained terpenoids,
saponins and leucoanthocyanins. From the antimicrobial study of disc diffusion
method, EA extract are the most active extract with the largest inhibition zone of 19
mm towards Erwinia sp. From the result of dot blot assay, EA extract is the most
antioxidative extract which showed the highest intensity of white colour compared to
the other extract. In the radical scavenging activity, standard ascorbic acid (IC50 9
µ.g/mL), exhibited the strongest scavenging activity compared with other extracts
followed with methanol extract (IC50 11 µ.g/mL), ethyl acetate (IC50 13 µ.g/mL) and
petroleum ether (IC50 42 µ.g/mL) extracts. While the DCM extract has high IC50
values of more than 100 µ.g/mL. This study has finally achieved its objectives by
focusing on screening phytochemicals, evaluating antioxidant and antimicrobial
properties from stem of E.spiralis.