Phytochemical screening and antimicrobial activities of strobilanthes crispus against selected bacteria / Nurfarahin Muhamad Kamarazzaman

Strobilanthes crispus also known as "Pokok pecah kaca" is a local herbs plant
belong to family Acanthaceae. Synthetic drug had given the drawback and herbs
plant can replaced the drug in term of medication. The leaves were selected
because of its variety benefits in traditional folk medicine to treat diabetes and
anticancer treatment This study was done to determine the antimicrobial activity
against Gram Positive bacteria which were Staphylococcus aureus and Bacillus
subtilis, and Gram Negative bacteria which were Klebsiella pneumoniae and
Pseudomonas aeruginosa. This study also wants to investigate the phytochemical
compound that present in leaves of S.crispus. The minimal inhibitory
concentration (MIC) also been carried out. The antimicrobial activity of S.crispus
leaves extract against selected bacteria was tested at different concentrations using
disc diffusion method. Identification of the phytochemical compound was
determined through phytochemical screening. In methanol extract, the highest
antimicrobial activities were at 400mg/ml concentration against P.aeruginosa
with 12.67 mm of inhibition zone while, the lowest was against K.pneumoniae
with 8.66 mm of inhibition zone at 200mg/ml concentration. The leaves extract of
S.crispus in hexane solvent give no inhibition zone at all concentration in this
study. The phytochemical screening showed positive result for the presence of
flavonoid and saponins in both leaves extract. The MIC for methanol extract was
determined at 25mglml against P.aeruginosa. In conclusion, S.crispus leaves
extract showed the best potential to inhibit P.aeruginosa at 400mg/ml
concentration. Further investigation should be conducted on other species of
bacteria using other part of this plant.