TLC-bioautographic method for detection of α-amylase inhibition in marine algae

Bioautographic method using TLC plays an important role in search of plant bioactive compound. In this study, TLC-bioautographic method was conducted to investigate antidiabetic activity in marine algae. Marine algae are abundant and known to have bioactive compound with health benefit including as antioxidant and antidiabetic effects. The objective of this study was to investigate the activity of a­ amylase inhibition and antioxidant in marine algae using TLC-bioautographic method. Marine algae samples were extracted in two different solvents, ethanol and ethyl acetate. After migration of the samples, the first plate was dipped in a-amylase solution and incubated. Then it was dipped in starch solution and incubated again. The reaction was visualized by washing the plate with Gram's iodine. Another developed plate was dipped in DPPH• solution. The ethyl acetate extracts of marine algae showed higher a­ amylase inhibition and antioxidant activity. Sample 8 showed the highest inhibition with moderate antioxidant activity. Antioxidant activity may contribute to a-amylase inhibition activity. Comparison of results may show relationship but must be further investigated. Marine algae showed the potential candidate for management of type 2 diabetes.