The antibacterial activity of Malaysian marine endophytic fungi fractions against gram positive bacteria

Marine endophytic fungi was proven to have a promising biological activity. Their unique and active metabolites lead to the research to search for a new potent bioactive metabolites. Most of the endophytes are capable in producing active metabolites such terpenoids, alkaloids, phenylpropanoids, aliphatic compounds and peptide. This study was conducted to determine the antibacterial properties of active :fractions from marine endophytic fungi, Graciliaria coronopifolia (CN), Graci/aria arcuata zanadini (MV) and Chaemotorpha minima (UFJ against the gram-positive bacteria (Staphylococcus aureus, Enterococcus faecalis, Bacillus subtilis, and Micrococcus luteus). These three seaweeds were collected from Teluk Kemang, Port Dickson and undergo extraction process by acetyl acetate extraction. The antibacterial activity against gram-positive bacteria was identified by conducting bioassay guided :fraction and antibacterial quick screenmg assay. Gram-positive infection contribute to disease such urinary tract infection, bacteremia, endocarditis, Intra-abdominal, wound infection and pelvic infections Current critical issue is the development of resistance against antibiotics. Commonly, two type of bacteria that keep develop resistance are Enterococcus faecalis and Staphylococcus aureus. Meanwhile from result obtained, all the active fractions of marine endophytic fungi were selectively against different bacteria. Graci/aria arcuata zanadini (MV) exhibited the highest bacterial inhibition percentage against S.aureus (79.2%). While Graciliaria coronopifolia (CN) highest percentage of inhibition against E. faecalis (53.6%). Further study need to be continued in order to isolate the potential active fractions of the marine endophytic fungi against gram-positive bacteria.