Synergistic effect of annona muricata ethanolic extract with cisplatin against breast cancer cell line (MDA­-MB-468)

Annona muricata has been used as a traditional medicine to as it has wide range of therapeutic effects. One of the uses of Annona muricata is it is used as anticancer. The aim of this study is to determine the inhibitory concentration by 50% cancer cell population (IC50) of Annona muricata and to assess the synergistic effect of Annona muricata when used with cisplatin. The cell viability was determined by using MTT assay. Cells were treated in triplicate with single drug and combined drug with varied concentrations. The result shows that the IC50 of the Annona muricata ethanol extract is 1640.3731 µM. The isoboligram and combination index (CI) value show there is a synergism between Annona muricata and cisplatin when the concentration of Annona muricata ethanol extract is fixed and the concentration of cisplatin is varied. The AMEE shows antiproliferative activity on breast cancer cell line MDA-MB-468, although the IC50 value is relatively high. Interestingly, AMEE shows synergistic effect when used in combination with chemotherapy agent, cisplatin.