Population dynamics and microbial biodiversity profiling of spontaneous fermentation of Carica papaya leaves

In this research, an aenarobic spontaneous fermentation of Carica papaya leaves has been conducted for 100 days. Ten sampling days has been collected in order to identify the microorganism present during the spontaneous fermentation. The maximum no. colonies (incfu/ml) estimated at each medium; Plate Counting Agar PCA, Man Ragosa Agar MRS, Dichloran Bengal Chloramphenicol (DRBC) agar, Man Rogosa Sharpe (MRS) agar and MacConkey MC. PCA, MRS and MC clarifies bacteria from yeast as yeast is preferable to grow on DRBC media. There are six genera of bacteria present in the ecosystem consist of Klebsiella, Cronobacteria, Pantoe, Pseudomonas, Bacillus and Enterobacter. A few types of Gram negative bacteria presented which are Pseudomonas, Salmonella and also Escherichia coli. Four genera of yeast and mould presented in the ecosystem which consist of Saccharomyces cerevisiae, Candida glabrata, Zygosaccharomyces and Aspergillus oryzae. Based on this research, acidic condition of the fermented juice optimize the growth of both bacteria and yeast. Yet, the growth of pathogens after day 45 should be avoided in order to prevent from harmful effect.