Microbiological analysis of Lactic Acid Bacteria (LAB) in naturally fermented Carica Papaya (CP) leaves

Conerlius, Carol Caroline (2016) Microbiological analysis of Lactic Acid Bacteria (LAB) in naturally fermented Carica Papaya (CP) leaves. [Student Project] (Unpublished)

Abstract

The present study reported on microbiological analysis of the natural fermentation of fresh CP leaves that involved isolation and cultivation of the microbes, colony counting, pH and gram staining. The microbes from naturally fermented CP leaves were isolated and cultivated on nutrient and MRS agars. The selective MRS agar used to cultivate LAB as suspected dominant bacteria which played preservative role during fermentation. Serial dilution method was used to ease the determination of number of colony presence on agar medium at different fermentation stages. The bio-preservative role of LAB was analysed through pH and its growth (colony counts). Low pH around 4.67 to 3.64 detected along fermentation process hinted the presence of LAB which inhibited the growth of other pathogenic bacteria. Gram staining results which indicated the presence of gram-positive bacteria further supported the hypothesis on the presence of LAB while colony counts on MRS medium recorded high number (between 4 to 10 million CFU/ml) These findings inferred the significant presence of LAB which could play preservative role in naturally fermented CP leaves.

Metadata

Item Type: Student Project
Creators:
Creators
Email / ID Num.
Conerlius, Carol Caroline
2012842446
Contributors:
Contribution
Name
Email / ID Num.
Advisor
So’Aib, Mohamad Sufian
UNSPECIFIED
Subjects: Q Science > QD Chemistry > Organic chemistry
T Technology > TP Chemical technology > Fermentation, Industrial
Divisions: Universiti Teknologi MARA, Shah Alam > Faculty of Chemical Engineering
Programme: Bachelor in Engineering (Hons.) Chemical and Bioprocess
Keywords: Fermentation, Carica papaya, Bio-preservation of CP leaves
Date: 2016
URI: https://ir.uitm.edu.my/id/eprint/118039
Edit Item
Edit Item

Download

[thumbnail of 118039.pdf] Text
118039.pdf

Download (377kB)

Digital Copy

Digital (fulltext) is available at:

Physical Copy

Physical status and holdings:
Item Status:

ID Number

118039

Indexing

Statistic

Statistic details