Study of therapeutic bioactives from Pandanus Amaryllifolius sp for functional food application / Nur Nadia Mohd Zakaria

The benefits and the nutraceutical values contained in the bioactive compounds are proven can be used as the therapeutic agents that have the potentials for prevention and cure human diseases. In this research, the extraction of bioactive compounds by using Pandanus Amaryllifolius sp leaves has been carried out to study the effect of microwave power level on extraction of bioactive compounds and also to determine the flavonoid compound and the antimicrobial properties of Pandanus Amaryllifolius sp. Traditional method which is soaking extraction is also conducted as comparison to microwave extraction which is the modern method. The HPLC analysis has been conducted for 30 minutes to detect the present of Catechin compound which is in flavonoid group and antimicrobial analysis for Pandanus Amaryllifolius sp has been investigated by using E.coli sp. From this research, it showed that there is present of Catechin compounds in the extract which is affected by the percentage concentration of solvent used and also the microwave power level used. Increase in microwave power level will enhance the percentage yield of Catechin compound. However, higher microwave power level and longer time of exposure to microwave radiation caused decrease in percentage yield of Catechin compound. This study also showed that the microbial activity of E.coli sp is retarded by the highly concentrated extract of Pandanus Amaryllifolius sp. Thus, microwave-assisted extraction offered a reliable and efficient method for extraction of bioactive compounds from Pandanus Amaryllifolius sp and the benefits and the nutraceutical values contained in the Pandanus Amaryllifolius sp is proven due to the present of bioactives compounds which can be used as the therapeutic agents that have the potentials for prevention and cure human diseases.