Cytotoxic effect of paclitaxel loaded and surface coated n-vinyl caprolactam / Normaizatul Shafiqah Zairosli

Paclitaxel alone has developed novel approach activity against breast cancer treatment. Highly effective antineoplastic activity of paclitaxel allows it to activate cell apoptosis and necrosis by stabilizing the formation of microtubules and thus prevent cell division. This study were intended to formulated new formulation which is a combination of Paclitaxel and N - Vinylcaprolactam ( NVC ) and compared the cytotoxic effects between Paclitaxel alone and new formulation of Paclitaxel and N - Vinyl Caprolactam against human breast cancer lines, MCF - 7. The method that had been conducted were Sulphorhodamine B assay (SRB assay) in order to determine cytotoxicity activity by evaluate the level of IC50. Based on the result, for IC50 Paclitaxel was 6 µg/ml meanwhile IC50 for newly formulated Paclitaxel and N - Vinyl Caprolactam was 10.3 µg/ml. Interestingly, although ICso for newly formulated Paclitaxel and N - Vinyl Caprolactam was slightly higher compared to IC50 of Paclitaxel but they do have significant roles in reducing percentages of cell viability.