Enzymatic extraction of hydrolyzed gelatin from Nemipterus japonicas (ikan kerisik) waste / Marommie Roland Mecilus

Enzymatic play an important role as to speed up the reaction for particular substances, and in this research the proteolytic enzymes were applied to extract the gelatin to become hydrolyzed gelatin. This research was used Nemipterus japonicas or locally known as "ikan kerisik" waste which is consisting of skin and bone was been exctacted to obtain the gelatin production. Two different enzymes which are alcalase and papain enzyme were used to extract the hydrolyzed gelatin and also combination of both enzymes was used in this study. The aim is to compose and identify optimum enzyme to produce highest yield and best quality of hydrolyzed gelatin. Meanwhile, in order to measure the amount of the production yield, the wet weight percentage and dry weight percentage together with the protein result percentage was been determined to calculate the highest yielding of hydrolyzed gelatin. Moreover, the quality of the hydrolyzed gelatin has determined due to its physiochemsitry which was devided into pH value, protein content and also the moisture content. Based on this study, combination of alcalase and papain enzymes had shown the highest yield and the best quality of gelatin then followed by alcalase enzyme and lastly the papain enzyme. The quality of the gelatin actually is still lack of information and it is recommended for the future study to include several indicators in proving the quality of the gelatin such as the anti-oxidant and the ability of water holding.