HPLC determination of vitamin C in commercial vitamin C tablets / Nurhusna Abu Bakar

In this study, HPLC technique was used for the quantitative determination of vitamin C in vitamin C tablets. The sample was pulverized and diluted to the diluting factor of 100 and 10. The samples were separated using C18 reversed-phase high performance liquid chromatography (HPLC) with mobile phase of deionised water and methanol (MeOH) (95:5% (v/v)) and mobile phase system with flow rate of 1.0 mL/min and UV detection at wavelength of 265 nm. Vitamin C peak was observed at retention time of approximately 2.126 minutes with total analysis time at about 3 minutes. The calibration curves were prepared for concentration; 0-200 ppm. The calibration curves shown good linearity as r=0.99965. Among five samples of tablets which was brand A, brand B, brand C, brand D and brand E, brand D was fully fulfilled the requirement which less 5% RSD and percent relative error within 10% of label claimed with 4.39% of RSD and 9.75% of relative error. The method was found to be efficient for the analysis of vitamin C in solid sample as it gave good accuracy excellent precision (low value of percent RSD (2.42%).