Fractionation and isolation of the Pandanus leaves extracts / Atiqah Akmal Azil

The main objective of this study was fractionation and isolation of compounds from n­ hexane extract of Pandanus leaves. First stage was fractionation of the n-hexane extract by using column chromatography. Fractionation was done to separate the number of compounds present in the extract. The compounds were purified by preparative Thin Layer Chromatography (TLC). The pure compound was subjected to Nuclear Magnetic Resonance (1H NMR) analysis to know the structure of the compound. We failed to deduce the structure of desired compound by using only of compound was not sufficient for 1H NMR because the quantity 13C-NMR and 20-NMR analysis. However, we did manage to identify several compounds by using TLC technique.