Effect of various receptor antagonists on the aqueous extract of dicranopteris linearis antinociceptive activity in mice / Nurul Husna Sodri

Dicranopteris linearis L, locally known as 'Resam' is traditionally used to control fever, to treat external wound, ulcers and broils. Scientifically, this plant has been proven to possess antinociceptive, anti-inflammatory and anti-pyretic activities. The present study was carried out to determine the involvement of various receptors in the antinociceptive activity of aqueous extract of Dicranopteris linearis (AEDL) using the mice abdominal constriction test. Mice were pre-treated subcutaneously with distilled water, followed 10 minute later by the subcutaneous (s.c) administration of 300, 500, and 1000 mg/kg concentration AEDL. All concentrations AEDL produced a significant (p<0.05) antinociceptive activity. Based on the antinociceptive profile, the 500mg/kg AEDL was chosen for further investigation. After 10 minutes s.c pretreatment with naloxone, methysergide, haloperidol, reserpine, theophylline, propanolol, pindolol, a-methyl-L­ tyrosine, p-phenylalanine, atropine, yohimbine or thioperamide, the mice were pretreated (s.c) with 500 mg/kg AEDL. The results obtained indicate that only 3 and 10 mg/kg naloxone, 5mg/kg atropine and yohimbine, and 10 mg/kg thioperamide significantly (p<0.05) reversed the extract antinociceptive activity, at the peripheral level, is mediated via modulation of the opioids, muscarinic, a-i-adrenoceptor and non-selective adenocine receptors systems. The statistical analysis was calculated by using one-way ANOVA.