Sub-acute toxicity of eugenia polyantha / Noor Nazira Hamat

The sub-acute toxicity of the aqueous extract of Eugenia polyantha (Myrtaceae) was studied in mice. In sub-acute toxicity studies, oral administration of 0.5 g/kg of Eugenia polyantha produced neither mortality nor changes in behaviour or any other physiological activities. No mortality was observed when a dose of 0.5 g/kg/day of aqueous extract of Eugenia polyantha extract were administered orally for a period of 28 days. In the blood chemistry analysis, no significant changes occurred in creatinine, blood urea nitrogen (BUN), aspartate transaminase (AST), alanine transaminase (ALT), total billirrubin, cholesterol, low density lipoprotein (LDL), triglycerides, cholesterol, creatinine kinase, total prothrombin and uric acid. Hematological analysis showed no differences in any of the parameters examined: white blood cell count (WBC), red blood cell count (RBC), haemoglobin concentration, mean corpuscular volume (MCV), mean corpuscular hemoglobin concentration (MCHC), neutrophils, lymphocytes, monocytes, eosinophils, basophils and thrombin in either the control or treated group. There were no significant difference in TBARS values in lung, liver, heart and kidneys of the mice in both control and treated groups. There were no significant differences in the body weight and relative organ weights between controls and treated animals.