In vitro micropropagation of musa acuminata cv. berangan (AAA) using new formulation of bioorganic media / Nur Atiqah Khirul Anuar

Plant tissue culture provides effective production for banana's high demand supply as one of subsistence crop and commercialized product. Commonly used plant tissue culture media such as Murashige and Skoog (MS), Gamborg's (B5) and Schenk and Hilderbrandt (SH) medium are known for their high mineral content and competent in promoting plant tissue culture. However, these media are costly, containing synthetic component and non-locally available. Therefore, this study has been conducted to develop an alternative plant tissue culture medium with high nutrient component. Fruits peel that have been formulated to develop media by using a fermentation process consisting of formula A (calamansi, key and kaffir lime peel), formula B (banana, papaya and pineapple peel), formula C (kaffir lime, apple mango and guava peel) and formula D (Banana, dragonfruit and honey mango peel) were prepared in different concentrations to study the effects of in vitro micropropagation of M.acuminata. The plantlets were cultured onto bioorganic media and incubated in culture room for six weeks. Number of shoots and height of the shoot were recorded. The highest number of shoot and root obtained in control media with 2.50± 0.56 and 3.17± 0.60 respectively while the highest plant height and number of leaves recorded in formula D with 2.78± 0.32cm and 2.83± 0.17 respectively. Contamination rate data showed the lowest in formula D with no contamination recorded. From this study, the application of bioorganic media is not significantly different (p>0.05) in promoting M.acuminata growth but significantly difference (p<0.05) in reducing contamination rate in M.acuminata. All bioorganic media formulas showed positive response in shoots regeneration. Therefore, this bioorganic media should be considered as the alternative culture media of M.acuminata as it showed positive response in promoting explants growth and it has low production cost as it is made up of organic waste and locally available.