Collection, extraction, fractionation and isolation of phytochemicals of pandanaceae species / Md Faizul Kamaruddin

The main objective of this study is to isolate as many as possible the phytochemical constituents of the Pandanus. There are several techniques involved in order to achieve the objective of this project. The first process involved the extraction of the sample by using petroleum ether and methanol. Further fractionation had been done to separate the number of compounds presented in the extract. The isolation process began when the promising compound(s) detected on the TLC plate. The preparative TLC method had been used to isolate the compound(s). The focus was on fraction 93 to 97 and fraction 120 to 122. 2 compounds were detected namely, compound! and i. The compound(s) had been sent for identification by using Nuclear Magnetic Resonance (1H NMR) spectrometer. Concisely, even though the compound(s) of desired has failed to be elucidated, we managed to identify the present of several potential compound(s) by using the TLC technique.