Isolation and characterization of endophytes from pandanus odoratissimus / Shafawati Shafie

Endophytes are fungi that colonize the inner part of the host plant for the whole or part of their life cycle. They are found in almost all plants in the earth. The purpose of this study is to isolate and identify the endophytes present in Pandanus odoratissimus, since there is no study has been done for that purposes. P. odoratissimus, a marine coastal plant was taken from Port Dickson, Negeri Sembilan. From this study, 45 endophytes have been isolated and most of the isolates were come from leaves samples. The morphological properties of 45 isolated endophytes were conducted macroscopically and microscopically. From the observation, there are 3 actinomycete appeared on the PDA medium. Through microscopic observation, all the isolated endophytes were determined by using Light Microscopy and picture was taken by using digital camera. From the observation, the 45 isolates were identified according to their hyphal characteristics and the asexual spore types and we found that 19 of the isolates having spores, 12 of isolates did not produce spores and the rest are not able to either be identified or observed under microscope.