Reproducibility comparative research of DNA extraction method performance towards animal based pharmaceutical and food products / Norhana Ghazali

Muslims are very concern to the Halal and Haram status of the products. The authentication of Halal and Haram in products needs thorough examination and sometimes the existing detection technique of products containing Sus scrofa DNA traces are not accurate and time consuming. In order to make the product examination more sensitive, rapid and reproducible, the optimized extraction method has to be developed. The developed DNA extraction method enables the detection of Sus scrofa DNA traces in not only food products but pharmaceutical products as well. This research has been conducted to determine the best DNA extraction method that enables extraction of Sus scrofa DNA traces with optimum yield and optimum purity. A number of samples of raw meat (beef, chicken and pork), processed food (sausage from different brands) and pharmaceutical product (toothpaste from different brands) were analyzed. DNA was extracted by three methods: (1) In-house DNA extraction method, (2) Commercial DNA extraction kit (Wizard Genomic DNA Purification Kit) and (3) DNAzol extraction method. Comparative study between those DNA extraction methodologies was done emphasizing on the performance of extraction yield and purity. The polymerase chain reaction (PCR) was applied to identify Sus scrofa DNA sequence. By using primer XK5, cytochrome b sequence specific for Sus scrofa could be identified by PCR. Primer XK5 was used so that the products showed species-specific DNA fragment of 125 bp from Sus scrofa. Identification of PCR product is possible by electrophoresis.