Identification of bacterial pathogens isolated from cockroaches at selected food premises in Shah Alam / Anis Husna Wahab @ Sulaiman

Cockroaches play an important role in transmission of different diseases either mechanically and biological because they spread and harbour various species of microorganisms. Escherichia coli is the ones of the gram – negative bacteria isolated from cockroaches. Escherichia coli are key player in bacterial species of faecal contaminations. The present study was carried out to identify the species of cockroaches commonly found in food premises and detect the presence of pathogenic bacterial harboured by the cockroaches. Screening of bacterial species on the cockroaches was performed using standard microbiological and biochemical test (RapID ONE System kit). Validation on the presence of Escherichia coli was conducted by PCR and sequencing. In the current study, two species of cockroaches were collected and identified as Periplanetta americana (American cockroach) and Blatta orientalis (Oriental cockroach).