Callus induction ability of roselle (Hibiscus sabdariffa L.) varieties Terengganu and UKMR-2 / Mizan Awab

Roselle is known as a multi-purpose plant as it provides many advantages such as for food, colorant and contains highly nutritional values including vitamin C, anthocyanin and hydroxycitric acid (HCA). In Malaysia, roselle has been considered as a relatively new crop and the production is not stable. Among problems faced by the farmers is the need of high quality roselle for better production. Nevertheless, natural and artificial hybridization are difficult to perform due to its cleistogamous nature of reproduction. Therefore, there is an urgent need to develop callus induction protocol for roselle so that it can be used as source materials to improve genetic material and regenerate a high quality roselle.