Detection of lipase gene of local soil bacteria isolated from domestic waste sites / Farhana Mohamad Izam Suhaimi

Lipases are important enzymes and known as most powerful biocatalyst in biotechnology industries. The aim of this study was to determine the lipase production from local soil isolates of domestic waste sites by using qualitative and quantitative methods, to identify the bacterial isolates that produced lipase via molecular methods and to detect the lipase gene segment from the chosen isolate with the highest lipase production. Six isolated lipase producing bacteria were selected and screened for their lipase activity. All isolates were isolated from two local domestic waste site soils at Shah Alam, Selangor namely garbage site and night market site.