In vitro regeneration of Orthosiphon stamineus by organogenesis / Siti Safinah Brahim

Orthosiphon stamineus or 'misai kucing' leaves have diuretic properties and has been utilized to expel uric corrosive stones from the kidneys because it provides rich medicinal compounds such as terpenoids, polyphenols and sterols. Due to the high demand of O. stamineus, this research is done with the reason to fulfill the demand and urgency from the consumers or from various industries to obtain the high quality product of O.stamineus. The aims of this study are to increase the number of individuals' plants and determine the sterilization methods of explants which different concentration of bleach was treated on explants. Couples drops of Tween 80 with the 5%, 10%, 15%, 20% and 25% sodium hypochlorite concentration of were used. In this experiment, explant from O. stamineus which are meristems, young leaves and young roots was put into Murashige and Skoog (MS) medium supplemented with BAP (6-Benzylaminopurine) and NAA (1-Naphthaleneacetic) and incubated at 25±2°C and 16/8 photoperiod hours. The growth of explant was observed within 6 weeks. The results of this study showed that the higher survival rate for explant is in 20% concentration of bleach treated for 20 minutes. Meanwhile, for the young roots and young leaves the best bleach concentration in sterilization process is 10% within 10 minutes. The healthy explant was subculture for 3 weeks and showed growth with higher number of leaves and the roots also lengthened rapidly. It is recommend increasing precautionary steps during sterilization process for example, sterilize the apparatus and materials that will be used that can lead to contamination.