Determination of gallic acid using different extraction solvents on Azadirachta indica / Nur Saffaaina Balqis Mahadi

This study was conducted to search for the bioactive compounds contain in the Azadirachta indica plant. The effectiveness of Azadirachta indica (neem) which aid in the treatment of inflammatory diseases is proven due to the bioactive compounds contains. Soxhlet extraction was used to extract the bioactive compounds from the leaves of Azadirachta indica using various solvents, which include ethanol, methanol, and aqueous (distilled water). High-performance liquid chromatography analysis was used to determine the phenolic content in Azadirachta indica. Based on the outcomes, the highest extraction yield was obtained by using aqueous (distilled water) solvent, which yielded 13.50%. While the highest concentration of gallic acid obtained from ethanol extraction, which is 38.02 ppm. The outcomes of this study provide a valid documentation and validation of the various biologically active compounds contain in Azadirachta indica plant which act as a natural remedy for a variety of ailments.