Cytotoxicity and DNA cleavage studies of salicylhydroxamic acid and its Ni(II), Cu(II) and Zn(II) complexes / Nor Haslini Wahid

Ni((II), Cu(II) and Zn(II) complexes of salicylhydroxamic acid have been synthesized
and characterized by elemental analysis, FT-IR, 1HNMR spectroscopy, magnetic
moment, melting point determination and conductivity measurement data. The spectral
data suggests that the salicylhydroxamic acid act as a bidentate ligand and is coordinated
to the metal ion through the hydroxamate group. The interaction of the salicylhydroxamic
acid ligand and its complexes with double stranded supercoiled DNA was studied using
agarose gel electrophoresis. From the DNA cleavage studies, it was found that the
supercoiled structure of the bacterial DNA was maintained indicating that all compounds
had no negative effect on bacterial DNA. The ligand and complexes were also subjected
to in vitro cytotoxicity test using Chang liver cells. Treatment of Zn(II) complex to these
cells have shown that the IC50 value is at 62 μM. This shows that Zn(II) complex had
toxic effect on Chang liver cells. A decrease in cells viability indicates that the cells were
stopping actively growing and dividing. But the ligand and other complexes tested were
found to be non-toxic.