Abstract
Eurycoma longifolia, locally known as Tongkat Ali is one of the popular herbs in
Malaysia and has been reported to contain various chemically active compounds. The
inconsistency in the composition of herbal products has always been a concern and thus, a
reliable method to ensure the quality of Eurycoma longifolia is crucial. In this study,
target compounds in Eurycoma longifolia were extracted using water by pressurised
liquid extraction (PLE), identified and quantified by online solid phase extraction-liquid
chromatography (SPE-LC). Optimization of PLE operating parameters conducted using
the response surface method (RSM) gave an optimum extraction temperature of 100 °C
and an extraction time of 30 minutes. For online SPE-LC with diode array detector
(DAD), 2 columns, polystyrene divinyl benzene (PSDVB) and Waters C18 were used. The
mobile phase compositions of water, methanol and acetonitrile and column switching
time for online SPE-LC were optimized. The developed method was used to analyse
Eurycoma longifolia roots from various sources and products from various manufacturers.
Selected chemometric techniques: cluster analysis (CA), discriminant analysis (DA) and
principal component analysis (PCA) were applied to the chromatographic dataset of 37
selected peaks. For root samples, CA revealed 3 clusters suggesting the classification of
quality based on the amount of selected compounds. PCA analysis applied to roots and
products clearly discriminated against the roots from products. CA gave 3 clusters of
roots and products with a 100 % correlation coefficient by DA. The clustering of
products in the same group with the roots may suggest that they were from the same
source. Pattern matching analysis showed no significant difference (p > 0.05) between the
roots and products within the same CA grouping with a percent similarity of 92.6 %. The
developed online SPE-LC method provides a fast and efficient approach in obtaining a
comprehensive fingerprint. The application of chemometrics to the fingerprint database
could be a promising approach for quality assessment of herbal formulation.
Metadata
Item Type: | Thesis (Masters) |
---|---|
Creators: | Creators Email / ID Num. Zaini, Nor Nasriah 2014445152 |
Contributors: | Contribution Name Email / ID Num. Thesis advisor Osman, Rozita (Prof. Assoc. Dr.) UNSPECIFIED |
Subjects: | Q Science > QD Chemistry > Extraction (Chemistry) |
Divisions: | Universiti Teknologi MARA, Shah Alam > Faculty of Applied Sciences |
Programme: | Master of Science (Chemistry) |
Keywords: | Eurycoma longifolia (Tongkat Ali); chemical component; chromatographic separation; quality control; herbal products |
Date: | August 2020 |
URI: | https://ir.uitm.edu.my/id/eprint/59875 |
Download
![[thumbnail of 59875.pdf]](https://ir.uitm.edu.my/style/images/fileicons/text.png)
59875.pdf
Download (2MB)
Digital Copy
![](/images/terminal.jpg)
Physical Copy
ID Number
59875
Indexing
![](https://library.uitm.edu.my/images/2020/01/23/googlescholar-01.png)
![](https://www.base-search.net/interface/images/base_logo_kl.png)