Antioxidant activity of isolated Flavonoids from the dried pericarp of Garcinia Parvifolia Miq. / Faizatul Isyraqiah Ahmad Muhammad

There are still many plants in this world with health benefits that have not been explored and commercialized, and Garcinia parvifolia is one of them. Currently, there are few reported studies done on the leaves, stem and root bark of Garcinia parvifolia Miq., but not much are concentrated on the fruits, especially the dried pericarp of the fruit. The purpose of this study is to isolate flavonoid compounds from dried fruit pericarp of Garcinia parvifolia Miq. and to determine the antioxidant property of the isolated flavonoid compounds. The samples were extracted using chloroform, hexane, ethyl acetate and methanol successively. The crude underwent column chromatography using toluene-ethyl acetate (50:50) yielding 71 fractions. After running through thin layer chromatography, it was discovered that 26 fractions contained flavonoids. The R f values ranging from 0.69 to 0.85 is suggested to be rutin while R f values ranging from 0.90 to 0.99 is suggested to be digoxin. The crude extract showed positive antioxidant activity, with the highest percentage of scavenging activity of 94.15% at 390mg/mL. A l l concentrations of crude extract have lower scavenging ability than the ascorbic acid control. The antioxidant activity of crude extract is 98.7% as good as ascorbic acid at different concentrations, while the isolated flavonoids antioxidant activity is between 74 to 98%. Rutin is the flavonoid most responsible for the antioxidant activity of the dried fruit pericarp of Garcinia parvifolia Miq. This study is hoped to help increase the commercialization of Garcinia parvifolia Miq.