The influence of 2, 4-dichlorophenoxy acetic acid concentration and light condition towards MR219, MR220 and MR297 rice varieties via in vitro / Muhammad Faiz Rosli, Wan Zuraida Wan Mohd Zain and Nur Atikah Zaharulil

The population of citizens in Malaysia relies heavily on the rice as their staple food for daily consumption. Currently. Malaysia has about 71.4% rice self-sufficiency level (SSL). Tissue culture of Malaysian rice is important in enhancing tissue culture protocol. Objective of the study is to study different 2,4-D hormone concentration effect and culture condition effect towards rice (Oryza Sativa L.) callus induction and plant regeneration. In this study, three varieties of rice was selected which are MR219. MR220 and MR297 with different 2. 4-D concentration (1.5mg/L,2.0mg/L and 2.5mg/L). The seeds were cultured in 16/8 h photoperiod and 24 hours dark condition. Three replications were done and the results were taken after four week. The average percentage for callus induction, roots and shoots initiation was calculated. Among this three sample. MR220exhibit highest frequency (66.67%) of callus induction in 16/8 h photoperiod at 2.5mg/L 2, 4-Dand MR219 highest frequency (66.67%) for 24 hours dark condition at 2.5mg/L 2. 4-D. Overall results indicated that the best callus frequency (66.67%) were induced when supplemented with2.5mg/L 2. 4-D in 16/8 h photoperiod for variety MR220.