Phytochemical screening and antioxidant activity of fresh stem of paederia foetida / Melur Asmida Mohd Hassan

This study is conducted to determine phytochemical constituent and antioxidant activity in various extracts of fresh stem of P. foetida. The extraction solvent used were ethanol, methanol and hexane. Antioxidant activity were quantified using total phenolic content, total flavonoids content and DPPH assay. Phytochemical screening confirmed the presence of saponin, phenolics, terpenoids, tannins, glycosides and alkaloids. Total phenolic contents were recorded at the range of 0.0111 mg GAE/g to 0.0399 mg GAE/g. The highest total flavonoid content was 0.0738 QE/g and lowest was 0.0172 QE/g for methanol and hexane extract respectively. Methanol showed highest antioxidant activity with IC50 = 1.50 mg/mL compared to others. Thus, this study proved that P. foetida can be used as one of natural antioxidants.