The optimization of the hydrolysis conditions (effect of temperature and pH) for the production of cutlassfish or “Ikan Timah” (Trichiurus Lepturus) hydrolysate / Noor Aqila Derahman

This study was done to determine the optimization of hydrolysis conditions in relation to the effect of pH and temperature on the production of Cuttlassfish hydrolysate by using alcalase. The analysis was done by obtaining the optimum percentage of nitrogen recovery and percentage of degree of hydrolysis after the Cuttlassfish was hydrolysed for 120 minutes and exposed to constant enzyme substrate ratio (2%). The production process of cuttlassfish hydrolysate includes homogenization, maintaining time and enzyme substrate ratio, adjust pH and temperature based on Response Surface Methodology (RSM), terminating enzyme, cooled, centrifuge, then the supernatant was collected to determine the percentage nitrogen recovery and degree of hydrolysis. Total nitrogen in supernatant was obtained using the Kjeldhal method and a-amino nitrogen was determined by formol titration method. All the data that were obtained from the analysis was analysed by employing the RSM using Design Expert 4 software. Model equation was proposed with proposed based on the effect of pH and temperature on the nitrogen recovery (NR) and degree of hydrolysis (DH). The optimum level for the percentage nitrogen recovery (%NR) at pH 4 and temperature 80°C is 1.30836 and desirability is 0.676. While for percentage of degree of hydrolysis at optimum level for pH 7.24, temperature 53.7°C is 4.41772 and desirability is 0.890.