Monitoring of aflatoxin M1 residues in milk marketed in Malaysia / Siti Nabilah Basar

Basar, Siti Nabilah (2014) Monitoring of aflatoxin M1 residues in milk marketed in Malaysia / Siti Nabilah Basar. [Student Project] (Unpublished)


Background: The purpose of this study is to investigate the occurrence of AFM1 in milk obtained from the market in Malaysia. A reliable, rapid and sensitive analytical method using liquid chromatography-tandem mass spectrometry (LC-MS/MS) method to determine the aflatoxin M1 in milk has been developed. Methods: Milk samples (69) were collected from supermarket around Puncak Alam city, Selangor state. Methodology includes simple extraction of sample by adding acetonitrile, mobile phase 4mM of ammonium acetate aqueous solution/ methanol (60:40, v/v), separation using Reverse Phase Symmetry C18 column and MS/MS analysis by using Multiple Reaction Monitoring (MRM) mode for quantitative analysis. The aflatoxin M1 LC­ MS/MS method was developed and validated according Commission Decision 2002/657/EC. Results: Among 69 samples, 3% of sample were found positive aflatoxin M1, with concentration level range of 0.036-0.045 ng/mL however, they were below maximum residue level (MRL) established by the European Community (EC). The limits of detection (LOO) and limits of quantifications (LOQ) were 0.01 ng/mL and 0.03 ng/mL respectively. The recovery values ranged from 86% to 93% when samples were fortified at three different concentrations. Conclusion: The method applied was successfully determined AFM1 in milk. The LOQs (0.03 ng/mL) of AFM1 detected was lower than the MRL for AFM1 in milk (0.05 ng/mL) set by the European Union. The method was sensitive, reliable, and suitable for residue confirmation analyses.


Item Type: Student Project
Email / ID Num.
Basar, Siti Nabilah
Email / ID Num.
Thesis advisor
Sameni, Mehdi
Subjects: R Medicine > RA Public aspects of medicine > Toxicology. Poisons
R Medicine > RA Public aspects of medicine > Public health. Hygiene. Preventive Medicine > Food and food supply in relation to public health > Foodborne diseases
Divisions: Universiti Teknologi MARA, Selangor > Puncak Alam Campus > Faculty of Health Sciences
Programme: Bachelor in Environmental Health and Safety
Keywords: Aflatoxin M1 Milk, Food safety, LC-ESI-MS-MS
Date: July 2014
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