Larvicidal activities of allamanda cathartica flowers extracts against aedes albopictus / Muhamad Aqil Arif Zaharinuddin

Mosquitoes borne disease are a public health burden causing high morbidity and mortality worldwide. In southeast Asia, Aedes species including Aedes albopictus are native to the tropical and subtropical climates. This secondary vector has the capacity to colonize and transmit a wide variety of arboviruses of medical and scientific importance. Temephos has long been used as a tool to eradicate mosquito vectors. Nevertheless, rampant uses of such chemical insecticides poses multiple issues of concern including resistance and toxicity which poses harm both to environment and the human health. This recent study was objectively to study the larvicidal activities of the Allamanda cathartica’s extract towards the late third and fourth instar larvae of Aedes albopictus. Maceration technique was used in order to extract the grounded flower part of the plant using the ethanol as solvent. According to World Health Organization protocol with minimum of 25 larvae were performed the larvicidal bioassays at five different concentration. Mortality of the larvae were recorded at 24 and 48 hours exposure and LC50 and LC90 values were calculated. Given that at 24 hours the LC50 and LC90 value were 578.649 ppm and 913.231ppm, while 48 hours showed that the results were LC50 at 385.154 ppm, and LC90 at 635.870 ppm. Significant results of the Allamanda cathartica’s extract showed that the plant have great potential to be substitute with the temephos. Extraction of Allamanda cathartica has a potential as a natural and eco-friendly larvicide agent. In future, studies could be establish on type of solvent correlate with test concentration to identify the most optimum Allamanda cathartica extraction as a larvicidal agent.