The antimitotic study of polygonum minus (daun kesum) leaves extract in different solvent / Noor Halimah Hamizi

Cancer is a disease that cause by the abnormal growth of cells that can invade in the body parts and spread to other organs. It is second disease that cause death. In 2015, 8.8 million death have been discovered by World Health Organisation (WHO). Nowadays, people are interested in medicinal herbs instead of synthetic medicines. Herbal medicine is a plant or mixtures of plant extracts which is used to treat illness and also to promote health. Medicinal herbs are inexpensive compared to synthetic medicine. The present study was done to determine the antimitotic activity of Polygonum minus by using different extraction at different concentration. Polygonum minus is prepared to extract in aqueous and methanol. Allium cepa is used for the treatment. The roots of Allium cepa was dip in the extraction. The root tips was cut to observe under microscope. It was dip in acetic acid and hydrochloric acid and stained with methylene blue. The mitotic index was calculated. Root tips cells in the extract shows minimum number of dividing cells compared to control group. Based on the mitotic index, aqueous has low mitotic index compared to methanol. Thus, aqueous has high cell division suppression. In the comparison of concentration, which involves 4 mg/ml and 8 mg/ml, it shows that 8 mg/ml has high cell division suppression compared to 4 mg/ml. This study shows that there is potential antimitotic activity of Polygonum minus extract.