Abstract
Fungus comprises of a diverse group of heterotrophs, where many are saprophytes that digest dead organic matter and organic wastes. Fungal contamination, however, can affect the structural integrity of a building and also affects the humans’ olfactory systems by mycotoxins. As herbal plants have antifungal properties such as tannins, terpenoids and flavonoids, this study aims to investigate the antifungal activity of Andrographis paniculata methanolic leaves extract against a fungus isolated and identified. Fungus was isolated from Final Year Project (FYP) Laboratory by air sampling onto Sabouraud Dextrose Agar (SDA). Agar plates were incubated in room temperature which is 28°C for about 3 to 7 days. Pure culture of fungus was presumptively identified using morphological (macroscopic) and microscopic characterization, where Lactophenol Cotton Blue Stain (LPCB) was used. The antifungal activity of methanolic leaves extract of Andrgraphis paniculata was evaluated on selected fungal isolate by using disc diffusion method at concentrations of 1000, 500, 250, 125, 62.5, 31.25 mg/mL respectively, that were diluted in DMSO (10%). Aspergillus spp. and Rhizopus spp. were presumptively identified by both macroscopic and microscopic characterization of two fungal isolates that grew on SDA. In other hand, no inhibitory effects were observed against Aspergillus spp. (selected fungus) using different concentrations of Andrographis paniculata methanolic leaves extract. The study showed that two species of fungi were isolated from FYP laboratory which were presumptively identified as Aspergillus spp. and Rhizopus spp. However, it is suggested to do molecular analysis for accurate confirmation of fungi. The Andrographis paniculata methanolic extract used in this study failed to express any antifungal activity probably because it was less stable.
Metadata
Item Type: | Thesis (Degree) |
---|---|
Creators: | Creators Email / ID Num. Misri, Anis Athirah 2013800934 |
Contributors: | Contribution Name Email / ID Num. Thesis advisor Mohamad Hussain, Roslinah UNSPECIFIED |
Subjects: | R Medicine > RA Public aspects of medicine > Toxicology. Poisons T Technology > TD Environmental technology. Sanitary engineering > Municipal refuse. Solid wastes |
Divisions: | Universiti Teknologi MARA, Selangor > Puncak Alam Campus > Faculty of Health Sciences |
Programme: | Bachelor of Medical Laboratory Technology |
Keywords: | Andrographis paniculata, Antifungal activity, Fungus, Fungal Identification, Puncak Alam. |
Date: | 2017 |
URI: | https://ir.uitm.edu.my/id/eprint/50074 |
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