Evaluation on anticoagulant activity on ethyl acetate extract from pericarp of parksia speciosa / Aisyah Zafirah Harun

Parkia speciosa also known as stink bean is usually found in tropical countries such as Malaysia, Indonesia, Thailand and Philippines. The seeds are usually consumed as condiment or vegetable with rice. The pericarp is usually separated from the seeds and discarded. Thus, the present study was carried out, using this waste to investigate whether it possess anticoagulant activity as some parts of P. speciosa were reported to have some beneficial values such as antioxidant, antidiabetic and antiangiogenic properties. The dried powder of pericarp was subjected to extraction using ethyl acetate via the maceration extraction method. Ethyl acetate was removed by rotary evaporation and the concentrated extract was prepared into a 1000 μg/ml stock solution using DMSO. The different concentrations of extract were prepared at 160 μg/ml, 80 μg/ml, 40 μg/ml, 20 μg/ml and 10 μg/ml using saline. Then, anticoagulant activity was evaluated by prothrombin time (PT), activated partial thromboplastin time (aPTT) and thrombin time (TT) using commercial control plasma. The statistical analysis was done by using One-way ANOVA and pos-hoc Dunnett’s tests. The results of the study showed a trend in which the coagulations time increased as the concentrations of ethyl acetate extract increases from 10 μg/ml to 160 μg/ml. However, only extract with concentration of 160 μg/ml showed significant effect on aPTT test (81.4 seconds, p<0.001) and TT test (88 seconds, p<0.05). Meanwhile, no significant effect was observed for PT test as p>0.05. The present study revealed that 160 μg/ml extract from pericarp of P. speciosa possessed anticoagulant activity. Hence, there is possibility that ethyl acetate extract could be a potential source of natural anticoagulant of blood coagulant disoders.