Determination of total phenolic content in water using UV-Vis spectrometry / Wan Nurfatin Nasuha Wan Ibrahim

Wan Ibrahim, Wan Nurfatin Nasuha (2016) Determination of total phenolic content in water using UV-Vis spectrometry / Wan Nurfatin Nasuha Wan Ibrahim. [Student Project] (Unpublished)


Phenolic content are most important contaminants exist in the surroundings. The release of phenol to the water is considered as a polluting chemical because it leads to several diseases if exposed to human being as well as animals if exceeded the allowable amount of TPC in water. A much more simple method with no usage of hazardous chemical which is chloroform, is use to determine TPC in water that is 4-aminoantipyrine method and was analysed using UV-Vis Spectrophotometer. The parameter for method validation are linearity, limit of detection, limit of quantification, repeatability, reproducibility as well as percent recovery. Calibration curve obtained from phenol standard range between 1 ppm to 7 ppm shows correlation coefficient of 0.9993 showing good linearity. Using this method, the LOD and LOQ obtained were 0.0159 0.001 mg L-1 and 0.257 0.0049 mg L-1. The repeatability is 1.84 % and the reproducibility is 11.61 %. The recovery test done were spiked with phenol at 1 ppm, 2 ppm, 5 ppm and 6 ppm and the range recovery were between 98 – 120 % where all the water samples were within the range.


Item Type: Student Project
Email / ID Num.
Wan Ibrahim, Wan Nurfatin Nasuha
Email / ID Num.
Thesis advisor
Ahmad, Nor Monica
Subjects: Q Science > Q Science (General) > Study and teaching > Laboratories. General works
Q Science > QD Chemistry
Divisions: Universiti Teknologi MARA, Negeri Sembilan > Kuala Pilah Campus > Faculty of Applied Sciences
Programme: Bachelor of Science (Hons.) Chemistry
Keywords: Determination, total phenolic content, water, uv-vis spectrometry
Date: 2016
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