Determination of total phenolic content in water using UV-Vis spectrometry / Wan Nurfatin Nasuha Wan Ibrahim

Phenolic content are most important contaminants exist in the surroundings. The release of phenol to the water is considered as a polluting chemical because it leads to several diseases if exposed to human being as well as animals if exceeded the allowable amount of TPC in water. A much more simple method with no usage of hazardous chemical which is chloroform, is use to determine TPC in water that is 4-aminoantipyrine method and was analysed using UV-Vis Spectrophotometer. The parameter for method validation are linearity, limit of detection, limit of quantification, repeatability, reproducibility as well as percent recovery. Calibration curve obtained from phenol standard range between 1 ppm to 7 ppm shows correlation coefficient of 0.9993 showing good linearity. Using this method, the LOD and LOQ obtained were 0.0159 0.001 mg L-1 and 0.257 0.0049 mg L-1. The repeatability is 1.84 % and the reproducibility is 11.61 %. The recovery test done were spiked with phenol at 1 ppm, 2 ppm, 5 ppm and 6 ppm and the range recovery were between 98 – 120 % where all the water samples were within the range.