Isolation of antioxidative constituents of gynura procumbens leaves (Sambung Nyawa) and its antibacterial potency against plant patogen / Nur Suhada Azman

Gynura procumbens known as “Sambung Nyawa” which is from Asteraceae
Family was used in this study to determine the antioxidative components and
antimicrobial properties. G. procumbens is a plant that contain active secondary
metabolites known as phytochemicals and commonly used as traditional medicine
to treat many types of diseases and illness. The leaves of G. procumbens was
extracted using three different polarities of solvents such as petroleum ether, ethyl
acetate and methanol and was analysed by phytochemical screening using several
spraying reagents to isolate the antioxidative constituents. The phytochemical
screening tests revealed the presence of tannins, saponins, triterpenoids and
flavonoids. The isolated compound from PE extract was labelled as S1 and
isolated compound from MeOH extract was labelled as S2. The isolated
compounds were further analysed by using FTIR and 1H NMR spectroscopy to
predict the structures. Based on the results, the S1 compound might be an alkaloid
glycoside and S2 compound might be a phenolic glycoside. As for the
antibacterial potency, the most effective extract is EA extract since it has the
biggest inhibition zone of 7 mm compared to other extracts.