Isolation and detection of Pseudomonas sp. in different type of soils using PCR method / Izzaz Hafiezy Zemry

Soil is considered to be one of the most common resource in the world and housed to many types of organisms such as plants and microorganisms. Different types and
conditions of the soils give and influence on the microorganisms that lives in soils as this might affect their nutrient uptakes and living condition. Among the
microorganisms that can be found in soil is Pseudomonas, a gram-negative bacterium that is known for its pathogenic characteristics. This study is aimed to isolate and detect the presence of Pseudomonas in different types of soils by using Polymerase Chain Reaction method. The study shows that Pseudomonas was successfully isolated from agriculture plantation, sewage planation, and also
playground soil. They are gram-negative and pathogenic bacteria. PCR method was used by amplifying the target DNA segment of the bacteria by using specific
primers. Results from the PCR process produce bands at the size between 200 bp and 300 bp which is met the expected results. Thus, it can be concluded that Pseudomonas bacterium does indeed lives in different types of soils and can be identify by using PCR methods.