Confirmation of Aeromonas sp., Streptococcus sp. and Vibrio sp. as suspected pathogen to Amphiprioninae sp. from Pusat Ikan Hiasan, Port Dickson / Nur Syahirah Shaari

There are many types of bacteria that are present in the universe as it plays crucial roles in the ecosystem. Bacteria are able to infects both humans and animals through variety type of transmission that leads to disease especially to ornamental fish. Ornamental fish are important for maintaining the diversity of the ecosystem while giving benefits to some of the marine creatures. Despite that, the organisms are facing real pathogenic infections such as bacterial, viral, fungal and parasitic infections. Amphiprioninae sp. is a type of marine creatures that are highly infected by bacteria that results in high mortality rate. The example of bacteria that are infecting Amphiprioninae sp. directly is Aeromonas sp., Streptococcus sp. and Vibrio sp. The purpose of this project is to confirm the types of bacteria that caused infections to the Amphiprioninae sp. and characterize them through Gram staining and biochemical tests. The final results of this project shows that Aeromonas sp., Streptococcus sp. and Vibrio sp. are absent in both samples. The samples are not infected by any of the bacteria predicted but are infected by other types of pathogenic bacteria. At the end of this project, the bacteria that were isolated from samples is Klebsiella pneumoniae from family Enterobacteriaceae. The biochemical tests are producing similar results to the predicted results of K. pneumoniae as it will produce results of positive methyl red, citrate utilization, catalase and negative indole and oxidase test.