Extraction and identification of active compounds from Christia Vespertilionis extracts by selected extraction techniques / Noradawiyah Mahayuddin

C. Vespertilionis known as red butterfly wings leaf has high potential in having active compounds that can be extracted which plays a crucial part in medicinal section and also being in a part of research. The objective of this study are to identify active compounds present in C. Vespertilionis using selected extraction techniques and to determine qualitatively phytochemical activities present in the C. Vespertilionis extracts. Maceration and supercritical-fluid-extraction (SFE) are chosen as the selected extraction techniques in hexane, chloroform, ethyl acetate and ethanol for maceration. The active compounds of the extracts were determined by using liquid chromatography mass spectrometry (LCMS) and Fourier transform infrared spectroscopy (FTIR). The antioxidant activity on the extracts were tested using free radical scavenging activity of 1,1-diphenyl-2-picrilhydrazyl (DPPH) assay as well as to determine phytochemical components, alkaloid and flavonoid using the suitable chemical tests. There were about more than 70 active compounds had been determined by using extract from SFE with ethanol co-solvent. The percentage of inhibition in antioxidant showed that ethyl acetate extract was the highest and SFE without ethanol co-solvent the lowest. Overall possible functional group that present in this plant were alkane, alkyl halide, aldehyde, alcohol and carbonyl. Alkaloid was present in all extracts meanwhile flavonoid only present in ethanol extract and SFE with and without co-solvent extracts. The maceration and SFE techniques contributed the same results but the time consumed and the ease of techniques were the differences between them.