Optimization of supercritical fluid extraction of Mariposa Christia Vespertillonis leaves to produce antioxidant components using response surface methodology / Suzana Tukiman

Mariposa Christia Vespertillonis (MCV) leaves or known as butterfly wing leaves are popular for traditional medicine treatment. The objective of this experiment is to optimize the antioxidant activity from MCV leaves extract using Supercritical Fluid Extraction (SFE) based on different temperature, pressure and particle size by apply Respond Surface Methodology (RSM). Besides that, it is also to evaluate the optimal condition of different extracting antioxidant activity from MCV leaves using design expert software 10. The condition range for both pressure and temperature are between 150- 350 bar and 30-70 °C with constant extraction time. The size of particles sample was between 60pm- 1 mm. Based on previous study, example of conventional methods of extraction are less effective compared to the latest which is favorable in industries because the extract obtained by SFE is higher in quality compared to conventional organic solvent extraction. The antioxidant is analyzed using UV-vis Spectrophotometer (UV-vis). Based on the calculated moisture content, the lowest and highest MC were 5.72% and 9.67%, respectively. The lowest antioxidant activity was 8.20% and higher antioxidant activity was obtained was 49.76%. Lower absorbance indicates higher free radical scavenging activities. Maximum percentage (%) indicated high scavenging activities, thus higher DPPH is good indication for antioxidant. The antioxidant activity was high with higher free radical scavenging abilities. The experimental optimal condition of antioxidant activity was 49.99 % and 50.30 % and predicted antioxidant was 50.29%. The optimization condition temperature was at 50°C, pressure at 282 bar and particle size at 500pm.