Antioxidant and antimicrobial activities of piper betle / Norsyahida Yasir

Piper betleleaves was extracted by soxhlet extraction method withhexane, chloroform and methanol solvent. The yield of crude extraction of hexane, chloroform and methanol is 9.53%, 13.47% and 7.86% respectively. All three types of crude extraction was undergo Thin Layer Chromatography (TLC) analysis to observed the presence of chemical constituent for each crude. Hexane crude was used dichloromethane;chloroform (3:2) as solvent system. Chloroform crude was used hexane;choroform with ratio 2:3 as a solvent system that produced several spot of chemical constituent of the TLC plate. Methanol crude was used solvent system of hexane;ethylacetate (4.5:0.5). All crude extracts was undergo microbial activity with gram positive bacteria Staphylococcus aureus, Bacillus subtilis and gram negative bacteriaEscherichia coliand Salmonella enterica. The crude has shown significant activity against bacteria Salmonella sp. with larger inhibition, followed by Bacillus subtilis, Staphylococcus aureus, and Escherichia coli. The crude also tested for antioxidant activity by DPPH Radical Scavenging method. The methanol crude extract gave highest percentage of inhibition of antioxidant rather than chloroform and hexane because the presence of phenolic compound.