Evaluation of antioxidants and antimicrobial potency of leaves of entada spiralis / Nor Nadiana Nordin

Entada spiralis also known as 'Akar sintok' which is from Leguminosea family
was used in this research to determine the antioxidant and antimicrobial
properties. This herbs plant was traditionally well known due the effectiveness to
treat disease and as health care. Generally, antioxidant is a valued substance needs
by organisms to trap the free radicals and avoid the aging process. Antimicrobial
or also known as antibiotics is an agent that can reduce and destroy the activity of
microorganisms such as fungi and bacteria. In determining these two properties,
several methods were conducted. The leaves of Espiralis was extracted using
three different polarity of solvents such as petroleum ether, dichloromethane and
ethyl acetate. The phytochemical screening on TLC with spraying reagent, dot
blot assay, UV and FTIR analysis was used as the method to determine the
antioxidant activity. The antimicrobial activity was determined by disc diffusion
method. The antioxidant properties were determined since the TLC plate shows
the positive result may due the presence of terpenoid compound. Besides, the
purple color of DPPH was immediately reducing to yellow spot during dot blot
assay. The extract also shows the antioxidant activity since it able to scavenge free
radical of DPPH at low concentration. The inhibition zone appeared on inoculated
agar plate indicated that the extract was active against the Erwinia Chysenthemi.
The extract has both properties of antioxidant and antimicrobial properties.
Among all extracts, the PE is the strongest extract to exhibit antioxidant while
DCM extract is the best to exhibit antimicrobial properties.