Determination of ascorbic acid in the multivitamin by differential pulse anodic stripping voltammetric technique at a glassy carbon electrode / Siti Norbaitina Shaari

Ascorbic acid cannot be synthesized by humans. This vitamin is rich in the varieties of
vegetables and fruits. Nowadays, ascorbic acid has been transformed into multivitamin
tablet which well-known as the dietary supplement. Growth in number of multivitamin
tablet production increases curiosity among the researcher whether the content of ascorbic
acid in the multivitamins dosage are in accordance with the standards and are safe to use
by consumer. The differential pulse anodic stripping voltammetry (DPASV) technique
using glassy carbon electrode (GCE) as the working electrode and phosphate buffer at pH
4.2 as the supporting electrolyte has been proposed to be developed. The experimental
voltammetric parameters were optimized in order to obtain a maximum response with
analytical validation of the technique. The optimum instrumental conditions for
electroanalytical determination of ascorbic acid by the proposed DPASV technique were
initial potential (Ei) = 0 V, final potential (Ef) = 0.7 V, accumulation time (tacc) = 60 s,
scan rate (v) = 0.125 VIs, accumulation potential (Eacc) = 0 V and pulse amplitude = 0.150
V. The curve was linear from 5 mg/L to 300 mg/L (R2=0.999) with detection limit of 0.25
mg/L. The precisions in terms of relative standard deviation (RSD) were 1.3%,0.5% and
0.06%, respectively on the same day precision. The recoveries achieved for ascorbic acid