Extraction and characterization of lipase from seed of arachis hypogaea l / Siti Nor Zulaiha Zakariya

Production cost of microbial lipase enzyme is high and industrial scale is still limited due
to high production costs. Seeds are commonly productive in triacylglycerols, which act as
compact source of energy for the fresh nascent plant. Therefore, seed lipase from Arachis
hypogaea L was introduced as biocatalyst due to low cost. Germinated and ungerminated
Arachis hypogaea L. seeds is use to determine the enzyme activity and selectivity by using
a mix substrate (coconut oil and cooking oil). Base-methylation and acid methylation
process were used to convert the free fatty acid (FFA) to fatty acid methyl ester before
analyze with gas chromatography (GC-MS). Particularly, the eight dominant fatty acids
in both seed show a change in percent of fatty acids after hydrolyze and it prove that the
enzyme in the both seeds are selective. Both of the seeds shows that their enzyme
selectivity is more toward oleic acid which have high percent change after hydrolyze.
Fatty acid shows the highest percentage in germinate seed compare to ungerminated seed.
Enzyme activity of peanut seed lipase show that the lipase more active in germinate seed
compare to ungerminated seed. Germinate seed lipase shows a great enzyme activity
because rate controlling of lipase in the middle of germination and the activity of the lipase
is great during that time.