Isolation of compounds from ethyl acetate crude extracts in stem bark of pithecellobium jiringa / Norshakireen Khairul Shahrom

Khairul Shahrom, Norshakireen (2016) Isolation of compounds from ethyl acetate crude extracts in stem bark of pithecellobium jiringa / Norshakireen Khairul Shahrom. [Student Project] (Unpublished)

Abstract

In this study, ethyl acetate (EA) crude extract of stem bark of Pithecellobium
jiringa was subjected to fractional isolation and proceeds with antifungal activity
determination. Five different EA fractions were successfully isolated and labelled
as FI, F2, F3, F4, and F5. The isolation of EA crude was achieved by using
column chromatography method and analysed by thin layer chromatographic
technique. Few compounds were identified based on TLC result on TLC plate.
However, the compounds give negative response to antifungal test, which means,
the compounds in the five fractions were not active towards antifungal test on T.
mentagrophyte and C. albicans. There is no inhibition activities reported towards
on these two fungi. The compounds then were been characterized by using GCMS
and IR spectrometer. The major compound is unsaturated fatty acid which is
hexadecanoic acid methyl ester.

Metadata

Item Type:	Student Project
Creators:	Creators Email / ID Num. Khairul Shahrom, Norshakireen UNSPECIFIED
Subjects:	Q Science > QD Chemistry > Organic chemistry Q Science > QD Chemistry > Extraction (Chemistry)
Divisions:	Universiti Teknologi MARA, Pahang > Jengka Campus > Faculty of Applied Sciences
Keywords:	Bark of pithecellobium jiringa, Antifungal activity determination, T. mentagrophyte, C. albicans, GCMS, IR spectrometer. Hexadecanoic acid methyl ester
Date:	2016
URI:	https://ir.uitm.edu.my/id/eprint/23082