Morphogenesis of capsicum frutescens linn. seed in rice water tissue culture media / Nor Hidayah Mohd Noor

Rice water contents variety of vitamins and minerals. The starch from the rice
water encourages the growth of beneficial soil bacteria to plant. The rice water
consist some amount of nitrogen, phosphorus and potassium that will enrich
mineral. Basic media that are frequently used in tissue culture include Murashige
and Skoog (MS) media. Reduction of culture media costs is continually targeted
in large-scale of MS media Therefore in this research, the effectiveness of rice
water usage as added supplement or as alternative nutrients resources for seeds
germination of Capsicum frutescens L. are studied. The objectives of this research
were to investigate the seed germination rate in different ratio of rice water in
basal MS media and to determine the rate of Capsicum frutescens L. growth in
rice water media with different concentration of Naphthaleneacetic acid (NAA)
and 6-Benzylaminopurine (BAP). In this research study had showed that seeds in
the treatment consist of rice water germinate faster rather than treatment without
rice water added. After 14 days, treatment E (100% rice water) and treatment B
(70% MS media and 30 % rice water) showed all the 60 seeds are germinated.
Among seeds in pillbox which consist of rice water and hormone of NAA and
BAP, amount of BAP 1.0 mg/l resulted with five seeds out often seeds were well
germinated compare to the other seeds exposed to NAA (0.5 mg/l), NAA (1.0
mg/l), and BAP (0.5 mg/l). In a conclusion, this study showed that rice water can
be the added as supplement to MS media and act as alternative nutrient sources
for the seeds to germinate. BAP hormone assists the shoot growth from the seeds